This concert, celebrating Willie Nelson's 90th birthday on April 29, spanned both April 29 and April 30 (Nelson was born on April 29, 1933, but his birth wasn't officially registered until April 30). It was not announced in advance which artists would perform on which date.

An edited concert film of this show was shown in theaters on June 11, 13, and 14, 2023, and broadcast on CBS on December 17, 2023. A CD/Blu-ray version, a streaming audio version, and an LP version were released on December 15, 2023.

4.29.23 setlist:
Whiskey River, Stay All Night (Billy Strings), The Party's Over (Charley Crockett), The Ghost (Particle Kid & Daniel Lanois), Remember Me (Edie Brickell & Charlie Sexton), Hello Walls (Lyle Lovett), I Can Get Off on You (Margo Price & Nathaniel Rateliff), Hands on the Wheel (Beck), Down Yonder, Funny How Time Slips Away (Norah Jones), Midnight Rider (Warren Haynes), Lovin' Her Was Easier (Than Anything I'll Do Again) (Roseanne Cash & Kris Kristofferson), Angel Flying Too Close to the Ground (Lukas Nelson), Night Life (Leon Bridges & Gary Clark Jr.), Texas Flood (Gary Clark Jr.), Willie Got Me Stoned and Took All My Money (Jack Johnson), Healing Hands of Time, Time of the Preacher (Tyler Childers & The Food Stamps), Still Is Still Moving to Me (Ziggy Marley), Opportunity to Cry (Tom Jones), Live Forever (Jamey Johnson), Blue Eyes Crying in the Rain (Bob Weir), Bloody Mary Morning (The Chicks), A Song for You (The Lumineers), City of New Orleans (Nathaniel Rateliff), I'd Have to Be Crazy (Sturgill Simpson), Mammas Don't Let Your Babies Grow Up to Be Cowboys (Miranda Lambert), Last Thing I Needed First Thing This Morning, Always on My Mind (Chris Stapleton), Long May You Run, For What It's Worth (Neil Young, Stephen Stills & Promise of the Real), Are There Any More Real Cowboys? (Willie Nelson, Neil Young, Particle Kid & Promise of the Real), Sing One with Willie, Pancho and Lefty (Willie Nelson & George Strait), Roll Me Up and Smoke Me When I Die (Willie Nelson & Snoop Dogg), On the Road Again (Willie Nelson Family), Will the Circle Be Unbroken, I'll Fly Away, Happy Birthday to You, Hard to Be Humble (everyone)

4.30.23 setlist:
Whiskey River, I Gotta Get Drunk (Billy Strings), Cowboys Are Frequently Secretly Fond of Each Other (Orville Peck), Yesterday's Wine (Charley Crockett), Seven Spanish Angels (Allison Russell & Norah Jones), Me and Paul (Dwight Yoakam & Benmont Tench), I Been to Georgia on a Fast Train (Margo Price & Waylon Payne), (Die When I'm High) Halfway to Heaven (Particle Kid & Daniel Lanois), It Ain't Over Yet, 'Til I Gain Control (Emmylou Harris & Rodney Crowell), Pancho and Lefty (Rosanne Cash), Night Life (Warren Haynes), My Heroes Have Always Been Cowboys (Lyle Lovett), Willie Got Me Stoned and Took All My Money (Jack Johnson), Blue Eyes Crying in the Rain (Beck), Across the Borderline (Tom Jones), Stay a Little Longer (Bob Weir), Good Hearted Woman (Shooter Jennings & Lukas Nelson), Angel Flying Too Close to the Ground (Lukas Nelson), Pick Up the Tempo, Heaven and Hell (The Avett Brothers), Pretty Paper (The Lumineers), [unknown] (Norah Jones), Help Me Make It Through the Night (Kris Kristofferson), A Song for You (Nathaniel Rateliff), Crazy (Sheryl Crow), Funny How Time Slips Away (Dave Matthews), The Maker (Emmylou Harris & Daniel Lanois), Georgia (on My Mind) (Jamey John son & Booker T. Jones), Highwayman (Lukas Nelson, Particle Kid, Shooter Jennings & Rosanne Cash), Stardust (Willie Nelson & Booker T. Jones), Far Away Places (Willie Nelson & Sheryl Crow), Will You Remember Me? (Willie Nelson & Lily Meola), Something You Get Through (Willie Nelson & Buddy Cannon), California Sober (Willie Nelson & Billy Strings), We Had It All, Live Forever (Willie Nelson & Keith Richards), On the Road Again, Will the Circle Be Unbroken, I'll Fly Away, Happy Birthday to You (everyone)